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rat anti dsred  (Proteintech)


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    Proteintech rat anti dsred
    Rat Anti Dsred, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+dsred/FITC+Anti-mouse+CD31/pmc07354059-93-40-43
    Average 95 stars, based on 1 article reviews
    rat anti dsred - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Ins1-Cre and Ins1-Cre ER Gene Replacement Alleles Are Susceptible To Silencing By DNA Hypermethylation
    Article Snippet: The following primary antibodies were used: polyclonal guinea pig anti-insulin (1:1000; Dako A0564 ( 40 )), rabbit anti-CREB (1:1000; Cell Signaling 9197 ( 41 )), rabbit anti-PCBP2 (1:1000; a gift from Dr. Stephen Liebhaber, University of Pennsylvania ( 42 )), rat anti-dsRed (1:1000; Chromotek, Planegg, Germany ( 43 )), and rabbit anti-Cre (1:2000; Novagen, Madison, WI ( 44 )).



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    Image Search Results


    (A) Diagram of experimental schematic. E8.5 timed pregnant mice received 1.5 mg of 4-OHT through oral gavage and were sacrificed at E10.5. (B) Anatomical structure of sagittal section. lv, lateral ventricle; di, diencephalon; 3v, third ventricle; PTh, prethalamus; CPa, caudal paraventricular nucleus area; Tu, tuberal region; Ahy, anterior hypothalamus. (C) Coronal sections corresponding to the line in (B). (D and E) Sagittal sections of Rax-CreER T2 ;Ai9 mice. vfb, ventral forebrain; mv, mesencephalic vesicle; 4v, fourth ventricle. (F and G) High power images of (C) and (E) respectively. (H) Coronal view of retina. OC, optic cup; LP, lens pit. Tdtomato signal was boosted with anti-DsRed immunolabeling. Red fluorescence exhibited mosaic pattern limited to ventral regions of the telencephalon and diencephalon (C–G), and as robust expression was detected in neuroretina (H). Sections were counterstained with DAPI (blue). Scale bars: 100 µm (C, F–H), 200 µm (D, E).

    Journal: PLoS ONE

    Article Title: Rax-CreER T2 Knock-In Mice: A Tool for Selective and Conditional Gene Deletion in Progenitor Cells and Radial Glia of the Retina and Hypothalamus

    doi: 10.1371/journal.pone.0090381

    Figure Lengend Snippet: (A) Diagram of experimental schematic. E8.5 timed pregnant mice received 1.5 mg of 4-OHT through oral gavage and were sacrificed at E10.5. (B) Anatomical structure of sagittal section. lv, lateral ventricle; di, diencephalon; 3v, third ventricle; PTh, prethalamus; CPa, caudal paraventricular nucleus area; Tu, tuberal region; Ahy, anterior hypothalamus. (C) Coronal sections corresponding to the line in (B). (D and E) Sagittal sections of Rax-CreER T2 ;Ai9 mice. vfb, ventral forebrain; mv, mesencephalic vesicle; 4v, fourth ventricle. (F and G) High power images of (C) and (E) respectively. (H) Coronal view of retina. OC, optic cup; LP, lens pit. Tdtomato signal was boosted with anti-DsRed immunolabeling. Red fluorescence exhibited mosaic pattern limited to ventral regions of the telencephalon and diencephalon (C–G), and as robust expression was detected in neuroretina (H). Sections were counterstained with DAPI (blue). Scale bars: 100 µm (C, F–H), 200 µm (D, E).

    Article Snippet: Tissue sections were fluorescently immunostained for the following markers: Tdtomato: 1∶500 rabbit polyclonal anti-DsRed (Living Colors), 1∶500 rat monoclonal anti-DsRed (Antibodies Online), neuronal markers: 1∶200 mouse monoclonal anti-HuC/D (Invitrogen), 1∶500 mouse monoclonal anti-NF165 (Developmental Studies Hybrididoma Bank), 1∶500 mouse anti-NeuN (Chemicon), tanycyte and/or glial progenitor cell markers: 1∶1000 rabbit polyclonal anti-GFAP (DAKO), 1∶1500 chicken polyclonal anti-vimentin (Millipore), 1∶500 rabbit polyclonal anti-Sox2 (Millipore), Müller glia markers: mouse monoclonal anti-P27 (Invitrogen), mouse monoclonal anti-GS (BD Bioscience), oligodendrocyte marker: 1∶500 rabbit polyclonal anti-Olig2 (Millipore).

    Techniques: Immunolabeling, Fluorescence, Expressing

    E10.5 pregnant mice received 1.5-OHT through oral gavage and were sacrificed at E12.5. (A–C) Coronal views of the anterior, medial and posterior hypothalamus, respectively. Tdtomato positive cells, visualized by anti-DsRed immunolabeling, co-express the neuronal markers NeuN and Hu (A, dashed square), as well as the neural progenitor marker vimentin (Vim) (B). A region of ventral hypothalamic neuroepithelium by the dashed square is enlarged to show the colocalization with neuronal markers in more detail (A, bottom left panel). A white asterisk indicates the dorsal hypothalamic domain of Tdtomato expression. (D and E) Coronal view of posterior pituitary and retina. Arrowheads indicate coexpression of Tdtomato with vimentin in optic nerve (ON), which contains axons of retinal ganglion cells (RGCs), an early-born cell type. 3V, third ventricle; PP, posterior pituitary; NR, neural retina; L, lens. Sections were counterstained with DAPI (blue). Scale bars: 100 µm.

    Journal: PLoS ONE

    Article Title: Rax-CreER T2 Knock-In Mice: A Tool for Selective and Conditional Gene Deletion in Progenitor Cells and Radial Glia of the Retina and Hypothalamus

    doi: 10.1371/journal.pone.0090381

    Figure Lengend Snippet: E10.5 pregnant mice received 1.5-OHT through oral gavage and were sacrificed at E12.5. (A–C) Coronal views of the anterior, medial and posterior hypothalamus, respectively. Tdtomato positive cells, visualized by anti-DsRed immunolabeling, co-express the neuronal markers NeuN and Hu (A, dashed square), as well as the neural progenitor marker vimentin (Vim) (B). A region of ventral hypothalamic neuroepithelium by the dashed square is enlarged to show the colocalization with neuronal markers in more detail (A, bottom left panel). A white asterisk indicates the dorsal hypothalamic domain of Tdtomato expression. (D and E) Coronal view of posterior pituitary and retina. Arrowheads indicate coexpression of Tdtomato with vimentin in optic nerve (ON), which contains axons of retinal ganglion cells (RGCs), an early-born cell type. 3V, third ventricle; PP, posterior pituitary; NR, neural retina; L, lens. Sections were counterstained with DAPI (blue). Scale bars: 100 µm.

    Article Snippet: Tissue sections were fluorescently immunostained for the following markers: Tdtomato: 1∶500 rabbit polyclonal anti-DsRed (Living Colors), 1∶500 rat monoclonal anti-DsRed (Antibodies Online), neuronal markers: 1∶200 mouse monoclonal anti-HuC/D (Invitrogen), 1∶500 mouse monoclonal anti-NF165 (Developmental Studies Hybrididoma Bank), 1∶500 mouse anti-NeuN (Chemicon), tanycyte and/or glial progenitor cell markers: 1∶1000 rabbit polyclonal anti-GFAP (DAKO), 1∶1500 chicken polyclonal anti-vimentin (Millipore), 1∶500 rabbit polyclonal anti-Sox2 (Millipore), Müller glia markers: mouse monoclonal anti-P27 (Invitrogen), mouse monoclonal anti-GS (BD Bioscience), oligodendrocyte marker: 1∶500 rabbit polyclonal anti-Olig2 (Millipore).

    Techniques: Immunolabeling, Marker, Expressing

    (A–H) Rax-CreER T2 ;Ai9 double heterozygote mice received 1.5 mg of 4-OHT at P28 and their pituitary glands were analyzed 3 days later. Coronal (A) and sagittal (B) sections stained with DsRed. Reporter expression was restricted in only pars nervosa (PN). PI, pars intermediate; PD, pars distalis. (C–F and G–H) High power confocal images of (A) and (B), respectively. A subset of Tdtomato-positive cells colocalized with vimentin (C, F), but not with GFAP or NF165. (I) Rax-CreER T2 ;Ai9 double heterozygote mice received 0.3 mg of 4-OHT three times at P5, P7, and P9, and their cerebellums were analyzed at P15. Reporter-positive cells were prominently robust in the granular layer (GL). (J) Rax mRNA in situ hybridization signal in cerebellum at P4.5 (data obtained from the Allen Brain Atlas), with the flocculus indicated by a black arrowhead. Sections were counterstained with DAPI (blue). ML, molecular layer. Scale bars: 200 µm (A–H), 400 µm (J).

    Journal: PLoS ONE

    Article Title: Rax-CreER T2 Knock-In Mice: A Tool for Selective and Conditional Gene Deletion in Progenitor Cells and Radial Glia of the Retina and Hypothalamus

    doi: 10.1371/journal.pone.0090381

    Figure Lengend Snippet: (A–H) Rax-CreER T2 ;Ai9 double heterozygote mice received 1.5 mg of 4-OHT at P28 and their pituitary glands were analyzed 3 days later. Coronal (A) and sagittal (B) sections stained with DsRed. Reporter expression was restricted in only pars nervosa (PN). PI, pars intermediate; PD, pars distalis. (C–F and G–H) High power confocal images of (A) and (B), respectively. A subset of Tdtomato-positive cells colocalized with vimentin (C, F), but not with GFAP or NF165. (I) Rax-CreER T2 ;Ai9 double heterozygote mice received 0.3 mg of 4-OHT three times at P5, P7, and P9, and their cerebellums were analyzed at P15. Reporter-positive cells were prominently robust in the granular layer (GL). (J) Rax mRNA in situ hybridization signal in cerebellum at P4.5 (data obtained from the Allen Brain Atlas), with the flocculus indicated by a black arrowhead. Sections were counterstained with DAPI (blue). ML, molecular layer. Scale bars: 200 µm (A–H), 400 µm (J).

    Article Snippet: Tissue sections were fluorescently immunostained for the following markers: Tdtomato: 1∶500 rabbit polyclonal anti-DsRed (Living Colors), 1∶500 rat monoclonal anti-DsRed (Antibodies Online), neuronal markers: 1∶200 mouse monoclonal anti-HuC/D (Invitrogen), 1∶500 mouse monoclonal anti-NF165 (Developmental Studies Hybrididoma Bank), 1∶500 mouse anti-NeuN (Chemicon), tanycyte and/or glial progenitor cell markers: 1∶1000 rabbit polyclonal anti-GFAP (DAKO), 1∶1500 chicken polyclonal anti-vimentin (Millipore), 1∶500 rabbit polyclonal anti-Sox2 (Millipore), Müller glia markers: mouse monoclonal anti-P27 (Invitrogen), mouse monoclonal anti-GS (BD Bioscience), oligodendrocyte marker: 1∶500 rabbit polyclonal anti-Olig2 (Millipore).

    Techniques: Staining, Expressing, In Situ Hybridization